|
KMID : 0358519920040020133
|
|
Korean Journal of Occupational and Environmental Medicine
1992 Volume.4 No. 2 p.133 ~ p.143
|
|
|
A Study on the Urinary Metabolites of Carbon Disulfide in Rats
|
|
Kim Chi-Nyon
|
|
|
Abstract
|
|
|
|
The objectives of this study are developing the optimal analytic methods for detecting urinary metabolites of carbon disulfide (CS2) and determining which metabolite is more useful biological indicator for CS2 metabolites. For this experiment, we used synthesized CS2 metabolites, 2-thio-thiazolidine-4-carboxylic acid (TTCA) and thiocarbamide. Those were identified by infrared spectroscopy and nuclear magnetic resonance spectroscopy. The maximum absorbances of TTCA and thiocarbamide were 272nm and 236nm respectively. Detection by UV detector was more precise and accurate than that by infrared spectrophotometer. Ethyl acetate extraction method was adopted as precleaning method for HPLC analysis of urinary methabolites. Urinary amount of TTCA and thiocarbamide were measured by HPLC after administration of CS2 (350mg/kg, 700mg/kg) into Sprague-Dawley rats intraperitoneally. Excreted amounts of urinary TTCA and thiocarbamide were increased by the doses of CS2 administration. The urinary amount of TTCA was greater than that of thiocarbamide after administration of both doses of CS2 in rats. There results showed that TTCA seened to be more useful biological indicator for CS2 exposure as ACGIH recommended, however further studies are need to simplyfy analytic methods for measuring the urinary metabolites of CS2 and clarify mechanisms of CS2 metabolism.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|